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Ki-67

Methodology

IHC

Specimen Requirements

To perform IHC testing, we request formalin-fixed, paraffin-embedded tissue samples. Please ensure that the blocks are clearly labeled with the patient name or identifier, date of birth and type of sample (tumor, normal). Additionally, a copy of the pathology report accompanying the tissue block will be very helpful. If tissue blocks are not available, we will also accept unstained slides (please submit 1-2 slides per antibody to be tested, cuts must be 4 microns thick).

Clinical Significance

Ki-67 is a nuclear protein that is expressed in proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. Increased proliferative activity is associated with more aggressive tumor and decreased disease-free survival period.

CPT Code(s)

88342 (qualitative IHC) or 88360 (quantitative/semi-quantitative – manual) or 88361 (quantitative/semi-quantitative – computer assisted) The listed CPT codes are for reference use only. These codes should not direct billing practices and may not accurately reflect current Clarient billing practices.

Turnaround Time

24 Hours

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