The Pulmotype Test

Pulmotype is a qualitative immunohistochemistry (IHC) assay consisting of a panel of five IHC monoclonal antibody reagents and an associated algorithm used for class assignment. It should be used on formalin-fixed, paraffin embedded lung cancer biopsies. It is indicated for use as an aid in the diagnosis of squamous versus adenocarcinoma histology for non-small cell lung cancer (NSCLC). The five Pulmotype biomarkers that make up the test are listed below along with a brief description of their potential function in the cell:
 

	CK5/6 recognizes cytokeratin 5 and 6 and is strongly differentially expressed in lung carcinoma subtypes.
	MUC-1 is a member of the mucin family; alleles of the MUC-1 gene are associated with different lung carcinoma subtypes.
	TRIM29 contains multiple zinc finger motifs and a leucine zipper motif and thus may act as a transcriptional regulatory factor.
	CEACAM5 is normally expressed in embryonic tissue and is aberrantly expressed in some cancers.
	SLC7A5 is involved in nutrient transport. Over-expression of SLC7A5 could help sustain the high growth rate of cancer cells by increasing a cell’s ability to consume nutrients.